Research Agendas
생물, 화학 유해 물질 분석 시스템 개발
Director
이규철
Period
2016.01.01 ~2018.12.31
Performer
최재원,김민정
테러란 정치적 또는 사회적 목적을 가진 개인이나 집단이 생물 무기나 화학 무기 등을 사용하여 계획적으로 어떤 목적을 달성하기 위해 행하는 위험 행위이다. 생물 무기는 세균, 바이러스 등 미생물과 독소 등을 의미하며 적군을 무력화시키거나 살상할 목적으로 사용되었다. 이러한 생물 무기에 의한 테러가 발생할 경우 국민의 안전에 심각한 위협이 될 수 있다. 이에 본 연구에서는 생물 작용제 분석 시스템 구축을 위해 생물 무기 4종, 즉 탄저균(Bacillus anthracis), 페스트균(Yersinia pestis), 브루셀라균(Brucella suis), 그리고 야토균(Francisells
tuleransis)을 선정하였다. 먼저 생물 무기 4종에 대한 분석법 개발 연구를 위해 미래창조과학부로부터 유전자 변형 생물체(LMO) 연구 시설 2등급 인증을 받았으며, 이를 근거로 질병관리본부로부터 각 세균에 대한 유전체를 분양받았다. 양성 대조군의 편의성과 실험간 교차 오염을 방지할 목적으로 각 유전자 증폭 개시자를 포함하는 유전자 벡터를 개발하였다. 생물 무기 4종의 각 유전자 2개 쌍에 대해 MIC real-time PCR을 사용하여 정확하게 4종을 구별하였으며, 검출 민감도는 0.01 ng/rxn까지 확보하였다. 향후 물 시료를 농축할 수 있는 장비를 휴대가 용이하도록 개선하는 연구 및 유전자를 현장에서 추출할 수 있는 시스템을 구축하는 연구가 이뤄져야 할 것이다. 아울러 본 연구에서 개발한 유전자 분석 키트를 실제 균체를 다룰수 있는 BL3등급 시설이 있는 질병관리본부나 화생방방호사령부 산하의 연구소 등의 협조를 받아 검증하는 연구가 필요하다.
목 차
제 출 문 ·····························································································································1
요 약 문 ·····························································································································6
목 차 ·····························································································································15
표 목 차 ·····························································································································17
그림목차 ·····························································································································18
제 1장 서 론 ·············································································································1
1.1 연구 배경 및 목적 ······························································································1
tuleransis)을 선정하였다. 먼저 생물 무기 4종에 대한 분석법 개발 연구를 위해 미래창조과학부로부터 유전자 변형 생물체(LMO) 연구 시설 2등급 인증을 받았으며, 이를 근거로 질병관리본부로부터 각 세균에 대한 유전체를 분양받았다. 양성 대조군의 편의성과 실험간 교차 오염을 방지할 목적으로 각 유전자 증폭 개시자를 포함하는 유전자 벡터를 개발하였다. 생물 무기 4종의 각 유전자 2개 쌍에 대해 MIC real-time PCR을 사용하여 정확하게 4종을 구별하였으며, 검출 민감도는 0.01 ng/rxn까지 확보하였다. 향후 물 시료를 농축할 수 있는 장비를 휴대가 용이하도록 개선하는 연구 및 유전자를 현장에서 추출할 수 있는 시스템을 구축하는 연구가 이뤄져야 할 것이다. 아울러 본 연구에서 개발한 유전자 분석 키트를 실제 균체를 다룰수 있는 BL3등급 시설이 있는 질병관리본부나 화생방방호사령부 산하의 연구소 등의 협조를 받아 검증하는 연구가 필요하다.
목 차
제 출 문 ·····························································································································1
요 약 문 ·····························································································································6
목 차 ·····························································································································15
표 목 차 ·····························································································································17
그림목차 ·····························································································································18
제 1장 서 론 ·············································································································1
1.1 연구 배경 및 목적 ······························································································1